mccA [2017-05-10 14:49:48]

O-acetylserine-thiol-lyase

locus: BSU27260

pI: 5.23

mw: 32.86 kDa

protein length: 307 aa

gene length: 921 bp

function: methionine-to-cysteine conversion

product: O-acetylserine-thiol-lyase

essential: no

synonyms: yrhA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]

Gene

Coordinates: 2,786,142 → 2,787,065

The protein

Catalyzed reaction/ biological activity

• O(3)-acetyl-L-serine + L-homocysteine = L-cystathionine + acetate (according to Swiss-Prot)

Protein family

• cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]
  description: [Pubmed|17056751]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|12642660,16885442], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

Biological materials

Mutant

• MGNA-A849 (yrhA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/849 NBRP B. subtilis, Japan]
• 1A945 ( ''mccA''::''kan''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A945&Search=1A945 BGSC]

References

• 17056751,16513748,16885442,12642660